This project consists of two strains: In the first the project aims to combine microbial taxonomic and functional properties with dietary factors.This is currently done by using the available metagenomic sequence data in 872 healthy controls, 223 patients with irritable bowel syndrome and 329 patients with inflammatory bowel disease and available data from FFQs. We are looking for (unsupervised) clusters of dietary patters and (unsupervised) clusters of microbiome features.
In the second strain, we are using data of the 10K metagenome project, wich is a sub-project within LifeLines where we aim to collect 10,000 full metagnomes of a cross-sectional cohort within the LifeLines population based study. This cohort will also be followed prospectively. We aim to perform similar analyses on the dietary patterns in relation to microbial features. Additionally, we will apply dietary indices to quantify the dietary variance and compute dietary quality scores. Nutrients analyses will be performed and the FOODTREE approach will be tested.
- Identifying dietary patterns and clusters of microbiome features in available metagenomic sequence data in 872 Healthy controls, 223 patients with irritable bowel syndrome and 329 patients with inflammatory bowel disease (Crohn’s disease and ulcerative colitis) and the available (processed) data from the FFQ.
- Within the 10K metagenome project we will question the Dutch Pathology database for the development of different types of cancer (including melanoma) during follow up and cancer and correlate this with the baseline microbial features of the 10K metagenomes.